Tag Archives: transgenic

Does anybody have a Cre-whatever mouse…?

By: Darryl Leja, NHGRI

By: Darryl Leja, NHGRI

Ever since I was a post-doc at Jax, I’ve been on the MGI mailing list. Some days it brings back memories.  Some days it brings laughter.  The other day I had a major problem with the spam filter because the discussion was about:  “Breeding from male with low sex drive” which, for obvious reasons, my mail filters thought was naughty.

But most often it is informative about research topics, mutant mice, and about resources that are useful either at MGI or other sites that mouse researchers like to use. Yesterday it was an announcement about a new segment of the MGI database: a Cre-Recombinase Portal.  One of the frequent questions on the list is “does anyone have a floxed mouse with _____ tissue expression….?” or “under control of ______ promoter….?”

The new portal will help researchers to find the right models.  The first part of the announcement mail (you’d have to go in and find Thursday’s mail; it has more details about how to us Creportal):

MGI has released a Cre Recombinase Data Portal (www.creportal.org) that specifically addresses the need to access cre expression and specificity data. Through this portal, users can access information about all existing cre transgenes and knock-ins. Data include the molecular description of the cre transgene or knock-in, the driver / promoter used, inducibility information, publications, and availability of cre mice through the IMSR. Detailed data, including annotated images showing cre activity / expression for the tissues analyzed are being added as available. Access to phenotypes displayed by cre-deleted mice is provided via integration with MGI’s phenotype data.

Currently, there are over 1,040 recombinase-containing transgenes or knock-in alleles cataloged.

Check it out if you or people you know need these mice.  Might save a lot of time if you can find the right mouse in the database rather than on the mailing lists….

Cre-Recombinase Portal: http://www.creportal.org/

Video Tip of the Week: A Mouse for All Reasons

At first the title of this paper made laugh, as I am a major fan of Paul Scofield’s performance in A Man for All Seasons.  And then I remembered what happened to Thomas More.  Well, the analogy drops away for me there…. A Mouse for All Reasons by the International Mouse Knockout Consortium presented the framework and foundations of the project to knock out every single protein-coding gene in mice, generate the corresponding ES (embryonic stem) cells, and make them available for development of subsequent transgenic mice.  Some of these mice will go on to give their life for science in a noble manner, I guess–so maybe the analogy picks back up :)

The project has made tremendous progress since that paper was published, and there are a lot of knockouts you should know about if you are interested in using mouse as a model organism.

For this tip of the week we’ll explore the new portal for the International Mouse Knockout Consortium (IMKC), which used to be at the URL for the KOMP, or Knock Out Mouse Project. It appears that the groups referenced in the Mouse for All Reasons paper have now harmonized on to the knockoutmouse.org site, and use a single portal for access to the information and reagents.  There are a variety of ways to search: browsing genes, specific text searching, and even a BioMart interface for the portal.  This short movie takes a look at those pieces to introduce you to the site.

The announcement for this came over the MGI mailing list as this:

The IKMC web portal

The International Knockout Mouse Consortium (IKMC) has launched its official web site at www.knockoutmouse.org, formerly the URL for the Knockout Mouse Project (KOMP). This extended site, supported by the NIH and EU, now serves as the common web portal for access to information on knockout vectors, ES cells and mice available from the international high-throughput knockout projects: KOMP, EUCOMM, NorCOMM and TIGM. Stay tuned for future enhancements as the content continues to evolve. We welcome your comments and feedback. (Please email to contact@knockoutmouse.org).

This site is maintained by the I-DCC and the KOMP-DCC

(http://www.knockoutmouse.org/about) . Supported by the European Union (Project number: 223592) and the National Institutes of Health (Grant number: NIH HG004074).

The International Mouse Knockout Consortium (2007). A Mouse for All Reasons Cell, 128 (1), 9-13 DOI: 10.1016/j.cell.2006.12.018

Extinct Genomes in PLOS One

ResearchBlogging.orgtasmanian tigerA paper published today in PLoS One reports on research that shows the feasibility of taking a gene or genomic region from an extinct species and inserting it into the genome of an extant species and resurrect the extinct species DNA function in the transgenic mice. The extinct species was the Tasmanian tiger or Thylacine (that links to the wikipedia page, anyone want to become the curator for the EOL page which is pretty minimal at this point?) and the ‘surrogate’ species was Mus musculus.

And, as the abstract says,

While other studies have examined extinct coding DNA function in vitro, this is the first example of the restoration of extinct non-coding DNA and examination of its function in vivo. Our method using transgenesis can be used to explore the function of regulatory and protein-coding sequences obtained from any extinct species in an in vivo model system, providing important insights into gene evolution and diversity.

It is an fascinating piece of research.

Continue reading