Tip of the Week: SIFT, Sorting (SNPs) Intolerant From Tolerant
Last tip I did was on PolyPhen, an algorithm that helps predict the phenotypic result of a non-synonymous SNP. There are other such algorithms available including MAPP (Multivariate Analaysis of Protein Polymorphism), SNPs3D and SIFT (Sorting Intolerant From Tolerant). It’s the latter that today’s tip will be briefly covering.
SIFT, like PolyPhen, has a web interface, but is also used in various other tools to give indication of an amino acid substitution affect on protein function. If you want to learn more about how the predictions are made (and some comparisons of the different methodologies), you might want to check out their paper (warning, pdf file).