Well, actually, GOEAST young scientist. This week’s tip of the week builds on the data from my previous tip. I had generated a list of genes and I wanted to use that list at a variety of sites to analyze the features of my list. So this week I have tried that at GOEAST.
I used the first 1999 items in my UniProt disease list and uploaded them to GOEAST. The movie shows you the process and a quick look at the outcome.
This view is just a quick example of a basic list upload using their Batch tool. The Batch tool algorithm is somewhat different from the pre-loaded microarray gene analysis they say, because of the way the background is calculated. It outputs the GO terms and groups the genes that fit that GO term. There are a number of other features of GOEAST that look intriguing and helpful. It looks like they handle various microarray platforms easily. They have a variety of outputs (web, text file, graphical). I couldn’t get the graphical output to work sometimes (probably my list is too large). I also would have liked to do the list the other way–to have the list of my genes and have the terms associated with them. I haven’t figured out if there is a way to do that so far.
I’m not going to draw many conclusions from this yet–I want to try a variety of tools and think about the features and the quality of the results. But this tool seemed to effectively group my genes into buckets with GO terms that could be helpful for an analysis.
Check out their paper for more details:
Zheng Q, Wang XJ. Nucleic Acids Res. 2008 May 16. GOEAST: a web-based software toolkit for Gene Ontology enrichment analysis. PMID: 18487275