We’ve long been fans of the tools developed by the team responsible for MINT: Molecular INTeraction database. MINT is a curated resource full of experimentally verified protein-protein interactions, with some great visualization options. In addition to the main MINT interface, there are other aspects to the site that bring other types of visualization as well. We have done a tip on MINT in the past, but we wanted to re-visit this for our SciVee collection, and also mention a handy tool called Connect. Connect can be used to enter a list of up to ~100 proteins and generate the connection map between them.
HomoMINT: this tool extends the experimentally-verified interaction collection to include inferred interactions for human, based on data from model organisms. So this is homologous interactions, hence the name….
Domino: a look at the domains that are involved in the protein-protein interactions.
VirusMINT: this aspect of MINT explores the viral proteins that includes how the virus proteins interact with host proteins to disrupt host physiology.
For this week’s tip I’ll focus mainly on the experimentally-verified portion of MINT and that interface, and introduce the others. You’ll see how to do a quick search, explore protein details, and then load up the network in the visualization tool. We have a full tutorial on MINT available for subscribers for people who want to go deeper into the functionality–we can only barely touch on the features in our screencast movie limit.
Edit: should have put the MINT link more clearly http://mint.bio.uniroma2.it/mint/Welcome.do Go to MINT.
Ceol, A., Chatr Aryamontri, A., Licata, L., Peluso, D., Briganti, L., Perfetto, L., Castagnoli, L., & Cesareni, G. (2009). MINT, the molecular interaction database: 2009 update Nucleic Acids Research, 38 (Database) DOI: 10.1093/nar/gkp983