Future of genome sequencing

We’ve written before about the feel of ‘a genome a day’ around here. RPM at Evolgen points to a paper that suggests his prediction (from last year) that “de novo sequencing of whole eukaryotic genomes may be a thing of the past.” Perhaps he is correct, though we do have quite a large number of de novo sequencing projects for eukaryotic genomes in the pipeline for the moment. He suggests that, as this paper has done, sequencing projects will “use 454 to sequence cDNA libraries.” Though there is loss of data in not sequencing the non-transcriptome part of the genome, as the abstract in the paper he points to says:

We conclude that 454 sequencing, when performed to provide sufficient coverage depth, allows de novo transcriptome assembly and a fast, cost-effective, and reliable method for development of functional genomic tools for nonmodel species. This development narrows the gap between approaches based on model organisms with rich genetic resources vs. species that are most tractable for ecological and evolutionary studies.

There is a lot of interesting discussion in the comments to his post.