I’m not a gene trap person, or even a mouse researcher, but I found this site recently and thought I’d pass it along to those of you who are. The site is The International Gene Trap Consortium (IGTC) site where you can search for cells by keyword, BLAST, expression, associated MeSH terms, or even biological pathways. Here’s the short description that they provide on their homepage:
Gene trapping is a high-throughput approach that is used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. In addition to generating standard loss-of-function alleles, newer gene trap vectors offer a variety of post-insertional modification strategies for the generation of other experimental alleles.
The International Gene Trap Consortium (IGTC) represents all publicly available gene trap cell lines, which are available on a non-collaborative basis for nominal handling fees. Researchers can search and browse the IGTC database for cell lines of interest using accession numbers or IDs, keywords, sequence data, tissue expression profiles and biological pathways.
I liked their background information on what GeneTraps were & what some of the pitfalls are with them. If you find anything good there, give me a ping (or comment) will ya? I always like to get feedback from ‘those in the know’. Thanks!